Abstract: The calibration method for the balloon-borne double-sphere electric field instrument, the micro-rocket-borne electric field instrument and the ground mill fieldmeter is introduced. The atmospheric electric field, relating with space physics and space environment, is a key parameter for studying various phenomena in earth science, such as lightning and earthquake, so the atmospheric electric field is becoming more and more important. With the lack of the national standards and military standards for the measurement and calibration of electric field, a set of calibration system is designed and developed to calibrate electric field instruments of different structures. The results are consistent with the theoretical calculation, and provides the foundation for the reliability of the electric field measurement data.