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空间毛细管式蛋白质结晶室样品加载技术研究

李娜 张贺桥 尚桂军 聂荣鑫 马建华 仓怀兴

李娜, 张贺桥, 尚桂军, 聂荣鑫, 马建华, 仓怀兴. 空间毛细管式蛋白质结晶室样品加载技术研究[J]. 空间科学学报, 2013, 33(4): 441-447. doi: 10.11728/cjss2013.04.441
引用本文: 李娜, 张贺桥, 尚桂军, 聂荣鑫, 马建华, 仓怀兴. 空间毛细管式蛋白质结晶室样品加载技术研究[J]. 空间科学学报, 2013, 33(4): 441-447. doi: 10.11728/cjss2013.04.441
LI Na, ZHANG Heqiao, SHANG Guijun, NIE Rongxin, MA Jianhua, CANG Huaixing. Study on Loading Techniques of Protein Species into Space Crystallization Chamber[J]. Journal of Space Science, 2013, 33(4): 441-447. doi: 10.11728/cjss2013.04.441
Citation: LI Na, ZHANG Heqiao, SHANG Guijun, NIE Rongxin, MA Jianhua, CANG Huaixing. Study on Loading Techniques of Protein Species into Space Crystallization Chamber[J]. Journal of Space Science, 2013, 33(4): 441-447. doi: 10.11728/cjss2013.04.441

空间毛细管式蛋白质结晶室样品加载技术研究

doi: 10.11728/cjss2013.04.441
基金项目: 载人航天工程应用系统项目资助
详细信息
    作者简介:

    仓怀兴, hxcang@ibp.ac.cn

  • 中图分类号: V527

Study on Loading Techniques of Protein Species into Space Crystallization Chamber

  • 摘要: 采用X-elax射线衍射技术研究蛋白质分子结构与功能的必要前提是获得高质量的蛋白质晶体.空间微重力环境是生长优质蛋白质晶体的理想场所.蛋白质样品的加载工艺对于空间蛋白质结晶实验的成效具有重要影响.针对为神舟八号飞船空间实验新研制的毛细管式空间蛋白质结晶室,结合样品加载基本流程,对加载工艺和伴随的气泡缺陷问题进行了系统和深入分析,确定了针头形状、毛细管封口质量和硅化效果、样品加载工具以及毛细管夹持方式等影响因素,并获得了实验测试验证.在此基础上,通过改进毛细管烧制工艺和样品加载工具,研制和使用专用毛细管夹具等措施,简化了蛋白质样品加载工艺,消除了气泡缺陷,提高了加载效率.新工艺的实施保证了空间实验任务的顺利完成.

     

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出版历程
  • 收稿日期:  2012-07-12
  • 修回日期:  2013-03-25
  • 刊出日期:  2013-07-15

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