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辐射条件下微藻基因表达内参基因的选择

蔡文凯 胡金璐 李双双 单革 王高鸿

蔡文凯, 胡金璐, 李双双, 单革, 王高鸿. 辐射条件下微藻基因表达内参基因的选择[J]. 空间科学学报, 2013, 33(6): 651-658. doi: 10.11728/cjss2013.06.651
引用本文: 蔡文凯, 胡金璐, 李双双, 单革, 王高鸿. 辐射条件下微藻基因表达内参基因的选择[J]. 空间科学学报, 2013, 33(6): 651-658. doi: 10.11728/cjss2013.06.651
Cai Wenkai, Hu Jinlu, Li Shuangshuang, Shan Ge, Wang Gaohong. Selection of Suitable Internal Control Genes in Microalgae Under Radiation Condition[J]. Journal of Space Science, 2013, 33(6): 651-658. doi: 10.11728/cjss2013.06.651
Citation: Cai Wenkai, Hu Jinlu, Li Shuangshuang, Shan Ge, Wang Gaohong. Selection of Suitable Internal Control Genes in Microalgae Under Radiation Condition[J]. Journal of Space Science, 2013, 33(6): 651-658. doi: 10.11728/cjss2013.06.651

辐射条件下微藻基因表达内参基因的选择

doi: 10.11728/cjss2013.06.651
基金项目: 国家自然科学基金项目(30970688)和载人航天项目共同资助
详细信息
    作者简介:

    单革,E-mail:shange@ustc.edu.cn;王高鸿 E-mail:ghwang@ihb.ac.cn

  • 中图分类号: V527

Selection of Suitable Internal Control Genes in Microalgae Under Radiation Condition

  • 摘要: 作为空间辐射的一种主要成分,紫外辐射可广泛引起陆地植物与水生生物细胞及其组份的破坏.荧光定量PCR技术广泛应用于各类胁迫环境下,研究目的基因的转录水平.在荧光定量PCR中选用合适的内参基因,能够更加准确地校正和标准化目的基因转录水平.本实验研究了辐射条件下水生生物莱茵衣藻6个传统内参基因18SrRNA,GAPDH,β-actin,β-tubulin,EF1-α和UBC基因表达的稳定性.经GeNorm软件研究分析,在辐射条件下,莱茵衣藻18SrRNA基因表达最不稳定,而选用β-actin和GAPDH作为双内参,可以得到更精确的实验结果.

     

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出版历程
  • 收稿日期:  2013-01-17
  • 修回日期:  2013-08-21
  • 刊出日期:  2013-11-15

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