Method and Technology of Monitoring the Expression of Flowering Gene Marked with Green Fluorescent Protein in Space Culture Chamber
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摘要: 绿色荧光蛋白（Green Fluorescent Protein，GFP）已被广泛用作一种强有力的生物发光报告蛋白.但是，其在空间植物培养箱中应用尚存很大困难.本文提出一种在空间植物培养箱中利用LED作为激发光源，对拟南芥中开花基因启动子控制下GFP基因表达的观察与分析方法，为在空间生物学实验中研究基因表达模式提供了新的方法和技术.Abstract: Green fluorescent Protein (GFP) has been widely used as a powerful bio-luminescence reporter molecule for monitoring gene expression on ground. However, it is still difficult to use GFP as a tool in the space culture chamber for life studies. In this study, we present a technical method, which use the LED as excitation light source, for monitoring and analysis of GFP gene expression of the flowering locus T gene in Arabidopsis thaliana that is controlled by the promoter. The method in this study provides a new technical reference for the gene expression studies in space.
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Key words:
- Space culture chamber /
- Green fluorescent protein /
- Flowering locus T /
- Arabidopsis
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