Volume 33 Issue 6
Nov.  2013
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Article Navigation >  Chinese Journal of Space Science  > 2013  >  33(6): 651-658
Cai Wenkai, Hu Jinlu, Li Shuangshuang, Shan Ge, Wang Gaohong. Selection of Suitable Internal Control Genes in Microalgae Under Radiation Condition[J]. Chinese Journal of Space Science, 2013, 33(6): 651-658. doi: 10.11728/cjss2013.06.651
Citation: Cai Wenkai, Hu Jinlu, Li Shuangshuang, Shan Ge, Wang Gaohong. Selection of Suitable Internal Control Genes in Microalgae Under Radiation Condition[J]. Chinese Journal of Space Science, 2013, 33(6): 651-658. doi: 10.11728/cjss2013.06.651
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Selection of Suitable Internal Control Genes in Microalgae Under Radiation Condition

doi: 10.11728/cjss2013.06.651
  • 1.

    Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072

  • 2.

    School of Life science, Chinese University of Science and Technology, Hefei 230022

  • 3.

    University of Chinese Academy of Sciences, Beijing 100049

  • Received Date: 2013-01-17
  • Rev Recd Date: 2013-08-21
  • Publish Date: 2013-11-15
    Abstract
  • As a major component of space radiation, ultraviolet radiation can extensively destruct terrestrial plants and aquatic. Real-time PCR technique has been widely used to detect the level of target mRNA expression when cell or tissues exposed to various types environmental stress. Choosing an appropriate internal control gene is important to accurately analyze the level of target gene transcription with real-time PCR technique. Therefore, our experiment tries to choose the most appropriate and stability reference gene from six traditional internal control genes, i.e., 18S rRNA, GAPDH, β-actin, β-tubulin, EF1-α and UBC in the Chlamydomonas reinhardtii under radiation conditions. The results showed that C. reinhardtii 18S rRNA gene expression is the most unstable through GeNorm analysis, but β-actin and GAPDH gene were finally selected as a pair of suitable internal control genes in expression analysis, with which more accurate experimental results can be obtained.

     

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