Abstract: Weightlessness is one of the important physical characteristics of space environment. For several decades, weightless environment in space has been used for investigation and exploration in many scientific fields. Because of shortage and limit of experiments in real space environment, many kinds of ground-based simulated techniques and methods inspired by space experimental environments were developed for simulating the weightlessness of space environment and the effects of weightlessness. However, these techniques and methods have limitations not only in principle, but also in application for space biology and biotechnology. In this paper, we introduced a new technique for simulating weightlessness, magnetic levitation of diamagnetic materials produced by large gradient high magnetic field, and summarized the research progress by using this technique in crystal growth of proteins, molecular cell biology and integrated biology.