Volume 33 Issue 3
May  2013
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Article Navigation >  Chinese Journal of Space Science  > 2013  >  33(3): 293-301
Zhang Yu, Hu Chunxiang, Zhang Delu. Effect of clinorotation on cellular structure, photosynthetic activity, carbohydrate and astaxanthin metabolism of Haematococcus pluvialis[J]. Chinese Journal of Space Science, 2013, 33(3): 293-301. doi: 10.11728/cjss2013.03.293
Citation: Zhang Yu, Hu Chunxiang, Zhang Delu. Effect of clinorotation on cellular structure, photosynthetic activity, carbohydrate and astaxanthin metabolism of Haematococcus pluvialis[J]. Chinese Journal of Space Science, 2013, 33(3): 293-301. doi: 10.11728/cjss2013.03.293
shu

Effect of clinorotation on cellular structure, photosynthetic activity, carbohydrate and astaxanthin metabolism of Haematococcus pluvialis

doi: 10.11728/cjss2013.03.293 cstr: 32142.14.cjss2013.03.293
  • 1.

    Key Laboratory of Fermentation Engineering, Minister of Education

  • 2.

    Hubei Collaborative Innovation Center of Industrial Fermentation

  • 3.

    College of Bioengineering, Light Industry Department of Hubei University of Technology, Wuhan 430068

  • Received Date: 2012-02-17
  • Rev Recd Date: 2012-10-15
  • Publish Date: 2013-05-15
    Abstract
  • During 20 days of simulated microgravity using clinostat (two-dimensional), the cellular structure, photosynthetic activity, primary and secondary metabolism of Haematococcus pluvialis were investigated. Results showed that the cell shape appeared abnormal and the volume became smaller than the ground control's. Ultrastructure analysis revealed that the starch grains seemed smaller and the thylakoid membranes were more randomly distributed and loosened after 20 days of clinorotation. Chlorophyll content decreased during the early period of clinorotation, but increased in the late phase. Carotenoid content and photosystem II efficiency decreased during the whole clinorotation. It could be presumed that the decreased photosynthetic efficiency might have some relation with the decreased pigment synthesis and the structure changes that happened on chloroplast after clinorotation. The smaller starch grains, together with the decreased starch content were probably due to an increased hydrolysis of amylase during clinorotation. The increased sucrose and trehalose played a protective role in the accommodation to early clinorotation. However, this led to a decrease of glucose and fructose utilized for the synthesis of protective disaccharides. In the middle phase of clinorotation (i.e. the acclimation period), a compensatory synthesis of chlorophyll, glucose and fructose was observed, whereas synthesis of sucrose and trehalose reduced. The decreased astaxanthin production during the whole clinorotation was due to the depressed carotenoid participating in the astaxanthin synthetic pathway.

     

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