Volume 36 Issue 4
Jul.  2016
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Article Navigation >  Chinese Journal of Space Science  > 2016  >  36(4): 562-565
WANG Lihua, XIE Junyan, ZHANG Yue, ZHENG Huiqiong. Method and Technology of Monitoring the Expression of Flowering Gene Marked with Green Fluorescent Protein in Space Culture Chamber[J]. Chinese Journal of Space Science, 2016, 36(4): 562-565. doi: 10.11728/cjss2016.04.562
Citation: WANG Lihua, XIE Junyan, ZHANG Yue, ZHENG Huiqiong. Method and Technology of Monitoring the Expression of Flowering Gene Marked with Green Fluorescent Protein in Space Culture Chamber[J]. Chinese Journal of Space Science, 2016, 36(4): 562-565. doi: 10.11728/cjss2016.04.562
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Method and Technology of Monitoring the Expression of Flowering Gene Marked with Green Fluorescent Protein in Space Culture Chamber

doi: 10.11728/cjss2016.04.562 cstr: 32142.14.cjss2016.04.562

  • Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032

  • Received Date: 2015-11-10
  • Rev Recd Date: 2016-05-09
  • Publish Date: 2016-07-15
    Abstract
  • Green fluorescent Protein (GFP) has been widely used as a powerful bio-luminescence reporter molecule for monitoring gene expression on ground. However, it is still difficult to use GFP as a tool in the space culture chamber for life studies. In this study, we present a technical method, which use the LED as excitation light source, for monitoring and analysis of GFP gene expression of the flowering locus T gene in Arabidopsis thaliana that is controlled by the promoter. The method in this study provides a new technical reference for the gene expression studies in space.

     

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