Volume 44 Issue 1
Feb.  2024
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Article Navigation >  Chinese Journal of Space Science  > 2024  >  44(1): 133-141 Open Access
XUAN Yingying, YANG Yutian, SUN Yuehong, CAI Nan, TANG Hanqin, LI Liang, CHEN Keming. Antagonistic Effects of Resveratrol on Reproductive Injury in Hind-limp Unloading Male Rats (in Chinese). Chinese Journal of Space Science, 2024, 44(1): 133-141 doi: 10.11728/cjss2024.01.2023-0063
Citation: XUAN Yingying, YANG Yutian, SUN Yuehong, CAI Nan, TANG Hanqin, LI Liang, CHEN Keming. Antagonistic Effects of Resveratrol on Reproductive Injury in Hind-limp Unloading Male Rats (in Chinese). Chinese Journal of Space Science, 2024, 44(1): 133-141 doi: 10.11728/cjss2024.01.2023-0063
shu

Antagonistic Effects of Resveratrol on Reproductive Injury in Hind-limp Unloading Male Rats

doi: 10.11728/cjss2024.01.2023-0063 cstr: 32142.14.cjss2024.01.2023-0063
  • 1.

    School of Pharmacy, Lanzhou University, Lanzhou 730000

  • 2.

    Basic Medical Laboratory of the 940 Hospital of Joint Logistic Support Hospital, Lanzhou 730050

  • Received Date: 2023-06-05
  • Accepted Date: 2024-01-15
  • Rev Recd Date: 2023-09-08
    • Available Online: 2023-12-04
    Abstract
  • To research the antagonistic effect of resveratrol on reproductive injury in hind-limp unloading male rats. The 30 experimental rats were randomly divided into placebo control group, hind-limp unloading group, and HU+Res group. The HU+Res group was given resveratrol at the dose of 30 mg·kg–1 respectively every two days, and the placebo control group and hind-limp unloading groups were given equal volume of physiological saline. After 28 days, all rats were executed, and testes and epididymis were taken for histopathology detection, tissue biochemical index detection, and protein expression analysis of testicular tissue. Compared with the placebo control group, the weight of testes and epididymis in the hind-limp unloading group was significantly reduced, and resveratrol could significantly improve these indicators. The results of histopathology showed that the number of seminiferous epithelium layers and the area of intercellular substance in the hind-limp unloading group were significantly reduced, with obvious edema, and the number of spermatogonium was significantly reduced. After treatment with resveratrol, the morphology of testicular tissue was partially restored. The detection results of oxidative stress and inflammatory factor levels showed that compared with the placebo control group, the hind-limp unloading group showed an imbalance in oxidative stress levels in the testicular tissue of rats, with a significant increase in inflammatory factor levels. After treatment with resveratrol, this harmful situation could be significantly reversed. Western blot results showed that resveratrol significantly reduced the expression levels of Bax in the testicular tissue of hind-limp unloading male rats, and increased the expression levels of Bcl-2, p-PI3K/PI3K, p-AKT/AKT. Conclusions resveratrol of 30 mg·kg–1 may exert a antagonistic effect on reproductive damage in hind-limp unloading male rats by activating the PI3K/AKT signaling pathway.

     

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