Volume 43 Issue 2
Mar.  2023
Turn off MathJax
Article Contents
Article Navigation >  Chinese Journal of Space Science  > 2023  >  43(2): 302-309 Open Access
HAN Pei, HOU Hongqu, FAN Yunlong, WANG Wenjia, LÜ Xuefei, ZHANG Wei, LI Xiaoqiong. Space Microbial Detection Method Based on Fluorescent LAMP (in Chinese). Chinese Journal of Space Science, 2023, 43(2): 302-309 doi: 10.11728/cjss2023.02.211125124
Citation: HAN Pei, HOU Hongqu, FAN Yunlong, WANG Wenjia, LÜ Xuefei, ZHANG Wei, LI Xiaoqiong. Space Microbial Detection Method Based on Fluorescent LAMP (in Chinese). Chinese Journal of Space Science, 2023, 43(2): 302-309 doi: 10.11728/cjss2023.02.211125124
shu

Space Microbial Detection Method Based on Fluorescent LAMP

doi: 10.11728/cjss2023.02.211125124 cstr: 32142.14.cjss2023.02.211125124
  • 1.

    Center for Space Science and Applied Research, Chinese Academy of Sciences, Beijing 100094

  • 2.

    School of Life, Beijing Institute of Technology, Beijing 100081

  • Received Date: 2021-11-24
  • Rev Recd Date: 2022-10-11
    • Available Online: 2023-04-08
    Abstract
  • Relevant studies on the International Space Station have shown that during the long-term operation of the space station, the existence of microorganisms may pose a threat to the long-term operation of the space station and stay of astronauts. Microbial species and content monitoring are important parts of microbial control in the station. Due to resource constraints such as volume and power consumption in space applications, detection methods based on lateral flow chromatography test strips and supporting handheld devices will be more suitable for daily monitoring of space microorganisms, combined with loop-mediated isothermal amplification and up-conversion luminescence characteristic spectra. Nucleic acid identification technology can meet the needs of rapid, sensitive and specific identification of microorganisms. In this research, aiming at the potential harmful microorganisms in the space for the living environment of astronauts and instrumentation equipment, the nucleic acid identification technology based on fluorescent LAMP (Loop-mediated isothermal Amplification) of the lateral flow chromatography test strip was developed. Research results showed that this technology can achieve high sensitivity, high specificity and rapid detection of common harmful microorganisms on the space station such as Escherichia coli and Staphylococcus aureus. The detection time is less than 60 minutes and the sensitivity can reach 100 copy·μL–1.

     

    • FullText(HTML)
  • loading
    • References(27)
  • [1]
    KAWAMURA Y, LI Y, LIU H, et al. Bacterial population in russian space station “Mir”[J]. Microbiology and Immunology, 2001, 45(12): 819-828 doi: 10.1111/j.1348-0421.2001.tb01321.x
    [2]
    BROCKETT R M, FERGUSON J K, HENNEY M R. Prevalence of fungi during Skylab missions[J]. Applied and Environmental Microbiology, 1978, 36(2): 243-246 doi: 10.1128/aem.36.2.243-246.1978
    [3]
    SIELAFF A C, URBANIAK C, MOHAN G B M, et al. Characterization of the total and viable bacterial and fungal communities associated with the International Space Station surfaces[J]. Microbiome, 2019, 7(1): 50 doi: 10.1186/s40168-019-0666-x
    [4]
    邹士文, 肖葵, 董超芳, 等. 空间站环境控制与生命保障系统微生物腐蚀行为与控制方法[J]. 科技导报, 2013, 31(30): 61-66 doi: 10.3981/j.issn.1000-7857.2013.30.009

    ZOU Shiwen, XIAO Kui, DONG Chaofang, et al. Corrosion behavior and control methods of microbiologically influenced corrosion of Space Station Environmental Control and Life Support System[J]. Science & Technology Review, 2013, 31(30): 61-66 doi: 10.3981/j.issn.1000-7857.2013.30.009
    [5]
    白子恒, 肖葵, 颜利丹. 空间站微生物腐蚀研究进展[J]. 中国材料进展, 2018, 37(1): 9-13 doi: 10.7502/j.issn.1674-3962.2018.01.02

    BAI Ziheng, XIAO Kui, YAN Lidan. Recently development of microbial corrosion of space station[J]. Materials China, 2018, 37(1): 9-13 doi: 10.7502/j.issn.1674-3962.2018.01.02
    [6]
    唐光泽, 白羽, 马欣新. TiO2光催化材料在未来空间领域的应用[C]//2009年空间环境与材料科学论坛论文集. 北京: 航天器环境工程编辑部, 2009: 178-181

    TANG Guangze, BAI Yu, MA Xinxin. Application of TiO2 photocatalytic materials in the future space field[C]//Forum on Space Environment and Material Science. Beijing: Editorial Department of Spacecraft Environmental Engineering, 2009: 178-181
    [7]
    YAMAGUCHI N, ROBERTS M, CASTRO S, et al. Microbial monitoring of crewed habitats in space—Current status and future perspectives[J]. Microbes and Environments, 2014, 29(3): 250-260 doi: 10.1264/jsme2.ME14031
    [8]
    KAROUIA F, PEYVAN K, POHORILLE A. Toward biotechnology in space: high-throughput instruments for in situ biological research beyond Earth[J]. Biotechnology Advances, 2017, 35(7): 905-932 doi: 10.1016/j.biotechadv.2017.04.003
    [9]
    ABAIBOU H, LASSEUR C, MABILAT C, et al. Current progresses of midass: microbial detection in air system for space[C]//40 th COSPAR Scientific Assembly. Moscow, Russia, 2014
    [10]
    ADAMSKI M G, GUMANN P, BAIRD A E. A method for quantitative analysis of standard and high-throughput qPCR expression data based on input sample quantity[J]. PLoS One, 2014, 9(8): e103917 doi: 10.1371/journal.pone.0103917
    [11]
    PARRA M, JUNG J, ALMEIDA E, et al. On-orbit quantitative real-time gene expression analysis using the Wetlab-2 system[C]//Annual Meeting of the American Society for Gravitational and Space Research. Alexandria, 2015
    [12]
    BURTON A S, STAHL S E, JOHN K K, et al. Off earth identification of bacterial populations using 16 S rDNA nanopore sequencing[J]. Genes, 2020, 11(1): 76 doi: 10.3390/genes11010076
    [13]
    SUN J D, SUN X L. Recent advances in the construction of DNA nanostructure with signal amplification and ratiometric response for miRNA sensing and imaging[J]. TrAC Trends in Analytical Chemistry, 2020, 127: 115900 doi: 10.1016/j.trac.2020.115900
    [14]
    JIA H X, LI Z P, LIU C H, et al. Ultrasensitive detection of microRNAs by exponential isothermal amplification[J]. Angewandte Chemie International Edition, 2010, 49(32): 5498-5501 doi: 10.1002/anie.201001375
    [15]
    ZHANG L R, ZHU G C, ZHANG C Y. Homogeneous and label-free detection of microRNAs using bifunctional strand displacement amplification-mediated hyperbranched rolling circle amplification[J]. Analytical Chemistry, 2014, 86(13): 6703-6709 doi: 10.1021/ac501645x
    [16]
    MORI Y, NOTOMI T. Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases[J]. Journal of Infection and Chemotherapy, 2009, 15(2): 62-69 doi: 10.1007/s10156-009-0669-9
    [17]
    GE Z L, LIN M H, WANG P, et al. Hybridization chain reaction amplification of microRNA detection with a tetrahedral DNA nanostructure-based electrochemical biosensor[J]. Analytical Chemistry, 2014, 86(4): 2124-2130 doi: 10.1021/ac4037262
    [18]
    DONG H F, ZHANG J, JU H X, et al. Highly sensitive multiple microRNA detection based on fluorescence quenching of graphene oxide and isothermal strand-displacement polymerase reaction[J]. Analytical Chemistry, 2012, 84(10): 4587-4593 doi: 10.1021/ac300721u
    [19]
    YEE E H, SIKES H D. Polymerization-based amplification for target-specific colorimetric detection of amplified Mycobacterium tuberculosis DNA on cellulose[J]. ACS Sensors, 2020, 5(2): 308-312 doi: 10.1021/acssensors.9b02424
    [20]
    YANG W , DANG X , WANG Q , et al. Rapid Detection of SARS-CoV-2 Using Reverse transcription RT-LAMP method. New York: Cold Spring Harbor Laboratory Press, 2020
    [21]
    LAMB L E, BARTOLONE S N, WARD E, et al. Rapid detection of novel coronavirus/Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by reverse transcription-loop-mediated isothermal amplification[J]. PLoS One, 2020, 15(6): e0234682 doi: 10.1371/journal.pone.0234682
    [22]
    HAGREN V, CONNOLLY L, ELLIOTT C T, et al. Rapid screening method for halofuginone residues in poultry eggs and liver using time-resolved fluorometry combined with the all-in-one dry chemistry assay concept[J]. Analytica Chimica Acta, 2005, 529(1/2): 21-25
    [23]
    LI Z, CHEN H, WANG P. Lateral flow assay ruler for quantitative and rapid point-of-care testing[J]. Analyst, 2019, 144(10): 3314-3322 doi: 10.1039/C9AN00374F
    [24]
    POSTHUMA-TRUMPIE G A, KORF J, VAN AMERONGEN A. Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey[J]. Analytical and Bioanalytical Chemistry, 2009, 393(2): 569-582 doi: 10.1007/s00216-008-2287-2
    [25]
    VARONA M, EITZMANN D R, ANDERSON J L. Sequence-specific detection of ORF1 a, BRAF, and ompW DNA sequences with loop mediated isothermal amplification on lateral flow immunoassay strips enabled by molecular beacons[J]. Analytical Chemistry, 2021, 93(9): 4149-4153 doi: 10.1021/acs.analchem.0c05355
    [26]
    JAWLA J, KUMAR R R, MENDIRATTA S K, et al. Paper-based Loop-mediated isothermal Amplification and Lateral Flow (LAMP-LF) assay for identification of tissues of cattle origin[J]. Analytica Chimica Acta, 2021, 1150: 338220 doi: 10.1016/j.aca.2021.338220
    [27]
    DU Y, POTHUKUCHY A, GOLLIHAR J D, et al. Coupling sensitive nucleic acid amplification with commercial pregnancy test strips[J]. Angewandte Chemie International Edition, 2017, 56(4): 992-996 doi: 10.1002/anie.201609108
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(6)

    Get Citation
    PDF
    XML

    Article Metrics

    Article Views(542) PDF Downloads(48) Cited by()
    Proportional views
    Related

    Journal Introduction

    ISSN 0254-6124       CN 11-1783/V

    Copyright © Editorial Office of Chinese Journal of Space Science.  京ICP备08100722号

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    x Close Forever Close

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return